更新時(shí)間:2016-06-03
我司所提供的實(shí)驗(yàn)KCL-22:慢性髓樣白血病細(xì)胞,不能用于人體實(shí)驗(yàn)和臨床診斷、治療。我司細(xì)胞研究中心承諾,盡Z大努力對(duì)實(shí)驗(yàn)細(xì)胞資源相關(guān)信息進(jìn)行及時(shí)更新,同時(shí)也保證純度在90%以上,咨詢購(gòu)買(mǎi)。
免費(fèi)咨詢:021-54720761
KCL-22:慢性髓樣白血病細(xì)胞培養(yǎng)方法:
收到細(xì)胞后,在倒置顯微鏡下觀察整個(gè)細(xì)胞生長(zhǎng)情況,如果細(xì)胞未長(zhǎng)滿,用75%酒精噴灑整個(gè)瓶消毒后放到超菌臺(tái)內(nèi),嚴(yán)格無(wú)菌操作,打開(kāi)細(xì)胞培養(yǎng)瓶,留10ml培養(yǎng)液繼續(xù)培養(yǎng)。
KCL-22:慢性髓樣白血病細(xì)胞初代培養(yǎng)原理:
將動(dòng)物機(jī)體的各種組織從機(jī)體中取出,經(jīng)各種酶(常用胰蛋白酶)、螯合劑(常用EDTA)或機(jī)械方法處理,分散成單細(xì)胞,置合適的培養(yǎng)基中培養(yǎng),使細(xì)胞得以生存、生長(zhǎng)和繁殖,這一過(guò)程稱原代培養(yǎng)。
選購(gòu)流程:
采購(gòu)實(shí)驗(yàn)裝備就是如此簡(jiǎn)單。我司細(xì)胞活力狀態(tài)良好的細(xì)胞株1420多株,細(xì)胞來(lái)源可靠,背景資料清晰,代數(shù)年輕,活力好。其中絕大部分是近年來(lái)從美國(guó)ATCC和NIH分批引進(jìn)的細(xì)胞種子,少部分來(lái)自全國(guó)各地細(xì)胞研究所。
從實(shí)驗(yàn)室的設(shè)計(jì)到實(shí)驗(yàn)過(guò)程中的操作,都需要嚴(yán)格控制無(wú)菌。由于細(xì)胞對(duì)于生長(zhǎng)的條件比較苛刻,所以無(wú)論從實(shí)驗(yàn)室的設(shè)計(jì),還是使用的物品及細(xì)胞培養(yǎng)的操作過(guò)程,都要注意保持無(wú)菌環(huán)境。
我司特性經(jīng)測(cè)試:具有良好的增殖和分化潛能,可分化為成骨細(xì)胞、脂肪細(xì)胞、軟骨細(xì)胞等。流式檢測(cè)結(jié)果顯示,CD29、CD44、CD73、CD105、CD166陽(yáng)性,CD11a、CD34、CD45陰性。細(xì)胞培養(yǎng)基其組成成分主要有:水、氨基酸、維生素、碳水化合物、無(wú)機(jī)離子及其他一些入核酸降解物、激素等。
我司其他相關(guān)批發(fā)產(chǎn)品展示:
Cs-(025)-003460 Bv-2細(xì)胞株/細(xì)胞,小鼠小膠質(zhì)瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003461 K6H6/B5細(xì)胞株/細(xì)胞,鼠雜交骨髓瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003462 RMC細(xì)胞株/細(xì)胞,大鼠腦膜細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003463 3T3/Fas/com細(xì)胞株/細(xì)胞,轉(zhuǎn)基因細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003464 CRL-1548細(xì)胞株/細(xì)胞,大鼠肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003465 Cyc-Tag(S49)細(xì)胞株/細(xì)胞,小鼠T淋巴細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003466 FDC-P1細(xì)胞株/細(xì)胞,小鼠骨髓細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003467 GT1-1細(xì)胞株/細(xì)胞,小鼠垂體瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003468 NG108-15細(xì)胞株/細(xì)胞,小鼠神經(jīng)母細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞與大鼠膠質(zhì)瘤細(xì)胞株/細(xì)胞之融合細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003469 PA12細(xì)胞株/細(xì)胞,小鼠胚胎成纖維細(xì)胞株/細(xì)胞(來(lái)自NIH3T3) ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003470 WEHI-3細(xì)胞株/細(xì)胞,小鼠血細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003471 MEL細(xì)胞株/細(xì)胞,小鼠紅白血病細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003472 SMC細(xì)胞株/細(xì)胞,兔主動(dòng)脈平滑肌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003473 AtT-20細(xì)胞株/細(xì)胞,小鼠垂體瘤細(xì)胞株/細(xì)胞(分泌促生長(zhǎng)激素分泌激素) ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003474 Fox-NY細(xì)胞株/細(xì)胞,小鼠骨髓瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003475 CCC-SMC-1細(xì)胞株/細(xì)胞,兔主動(dòng)脈平滑肌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003476 CCC-SMC-2細(xì)胞株/細(xì)胞,兔主動(dòng)脈平滑肌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003477 MMQ細(xì)胞株/細(xì)胞,大鼠垂體瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003478 BaF3細(xì)胞株/細(xì)胞,小鼠原B細(xì)胞株/細(xì)胞株 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003479 BC3H1細(xì)胞株/細(xì)胞,小鼠腦瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003480 IEC-6細(xì)胞株/細(xì)胞,大鼠小腸隱窩上皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003481 LLC-PK1細(xì)胞株/細(xì)胞,豬腎細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003482 3T6-Swiss albino細(xì)胞株/細(xì)胞,小鼠胚胎成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003483 C3H 10T1/2 2A6細(xì)胞株/細(xì)胞,小鼠成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003484 DCS細(xì)胞株/細(xì)胞,小鼠樹(shù)突狀細(xì)胞株/細(xì)胞肉瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003485 IAR20細(xì)胞株/細(xì)胞,大鼠肝細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003486 NBLE細(xì)胞株/細(xì)胞,新生牛眼晶體上皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003487 NBTF細(xì)胞株/細(xì)胞,新生牛眼Tenon's囊成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003488 RCBBF細(xì)胞株/細(xì)胞,兔角膜后基質(zhì)層成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003489 RCFBF細(xì)胞株/細(xì)胞,兔角膜前基質(zhì)層成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003490 RTE細(xì)胞株/細(xì)胞,大鼠氣管上皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003491 RYTF細(xì)胞株/細(xì)胞,兔眼Tenon's囊成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003492 CCC-Ca761-03細(xì)胞株/細(xì)胞,小鼠癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003493 Hed68細(xì)胞株/細(xì)胞,菓子貍腎上皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003494 L6細(xì)胞株/細(xì)胞,大鼠骨骼肌成肌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003495 LA795細(xì)胞株/細(xì)胞,小鼠肺腺癌細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003496 MV-1-Lu細(xì)胞株/細(xì)胞,鼬肺上皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003497 N/N1003A(RLE)細(xì)胞株/細(xì)胞,兔晶體上皮細(xì)胞株/細(xì)胞永生系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003498 RBL-1細(xì)胞株/細(xì)胞,大鼠嗜堿性粒細(xì)胞株/細(xì)胞性白血病細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003499 SH2細(xì)胞株/細(xì)胞,小鼠B淋巴細(xì)胞株/細(xì)胞雜交瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003500 SH3細(xì)胞株/細(xì)胞,小鼠B淋巴細(xì)胞株/細(xì)胞雜交瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003501 145-2C11細(xì)胞株/細(xì)胞,倉(cāng)鼠/小鼠雜交瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003502 Cl.Ly1+2-/9細(xì)胞株/細(xì)胞,小鼠T淋巴細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003503 IR983F細(xì)胞株/細(xì)胞,大鼠骨髓瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003504 J774A.1細(xì)胞株/細(xì)胞,小鼠單核巨噬細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003505 M-1細(xì)胞株/細(xì)胞,小鼠腎集合管細(xì)胞株/細(xì)胞(SV40轉(zhuǎn)化) ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003506 MDCK superdome細(xì)胞株/細(xì)胞,狗腎細(xì)胞株/細(xì)胞隆突型 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003507 MFC-GFP細(xì)胞株/細(xì)胞,綠色熒光蛋白標(biāo)記小鼠前胃癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003508 Mouse podocyte細(xì)胞株/細(xì)胞,小鼠腎足細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003509 U14細(xì)胞株/細(xì)胞,小鼠子細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003510 U14-GFP細(xì)胞株/細(xì)胞,綠色熒光蛋白標(biāo)記小鼠子細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003511 MPC-11細(xì)胞株/細(xì)胞,小鼠漿細(xì)胞株/細(xì)胞瘤 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003512 15P-1細(xì)胞株/細(xì)胞,轉(zhuǎn)PYTL基因小鼠支持細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003513 BNL1ME A.7R.1細(xì)胞株/細(xì)胞,BALB/C小鼠肝上皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003514 CT26.WT[CT26WT]細(xì)胞株/細(xì)胞,小鼠結(jié)腸癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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