更新時(shí)間:2016-06-03
我司所提供的實(shí)驗(yàn)Ketr-3:人腎癌細(xì)胞,不能用于人體實(shí)驗(yàn)和臨床診斷、治療。我司細(xì)胞研究中心承諾,盡Z大努力對(duì)實(shí)驗(yàn)細(xì)胞資源相關(guān)信息進(jìn)行及時(shí)更新,同時(shí)也保證純度在90%以上,咨詢購(gòu)買。
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Ketr-3:人腎癌細(xì)胞培養(yǎng)方法:
收到細(xì)胞后,在倒置顯微鏡下觀察整個(gè)細(xì)胞生長(zhǎng)情況,如果細(xì)胞未長(zhǎng)滿,用75%酒精噴灑整個(gè)瓶消毒后放到超菌臺(tái)內(nèi),嚴(yán)格無菌操作,打開細(xì)胞培養(yǎng)瓶,留10ml培養(yǎng)液繼續(xù)培養(yǎng)。
Ketr-3:人腎癌細(xì)胞初代培養(yǎng)原理:
將動(dòng)物機(jī)體的各種組織從機(jī)體中取出,經(jīng)各種酶(常用胰蛋白酶)、螯合劑(常用EDTA)或機(jī)械方法處理,分散成單細(xì)胞,置合適的培養(yǎng)基中培養(yǎng),使細(xì)胞得以生存、生長(zhǎng)和繁殖,這一過程稱原代培養(yǎng)。
選購(gòu)流程:
采購(gòu)實(shí)驗(yàn)裝備就是如此簡(jiǎn)單。我司細(xì)胞活力狀態(tài)良好的細(xì)胞株1420多株,細(xì)胞來源可靠,背景資料清晰,代數(shù)年輕,活力好。其中絕大部分是近年來從美國(guó)ATCC和NIH分批引進(jìn)的細(xì)胞種子,少部分來自全國(guó)各地細(xì)胞研究所。
從實(shí)驗(yàn)室的設(shè)計(jì)到實(shí)驗(yàn)過程中的操作,都需要嚴(yán)格控制無菌。由于細(xì)胞對(duì)于生長(zhǎng)的條件比較苛刻,所以無論從實(shí)驗(yàn)室的設(shè)計(jì),還是使用的物品及細(xì)胞培養(yǎng)的操作過程,都要注意保持無菌環(huán)境。
我司特性經(jīng)測(cè)試:具有良好的增殖和分化潛能,可分化為成骨細(xì)胞、脂肪細(xì)胞、軟骨細(xì)胞等。流式檢測(cè)結(jié)果顯示,CD29、CD44、CD73、CD105、CD166陽性,CD11a、CD34、CD45陰性。細(xì)胞培養(yǎng)基其組成成分主要有:水、氨基酸、維生素、碳水化合物、無機(jī)離子及其他一些入核酸降解物、激素等。
我司其他相關(guān)批發(fā)產(chǎn)品展示:
Cs-(025)-003309 PC-12細(xì)胞株/細(xì)胞,大鼠腎上腺嗜鉻細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞(低分化) ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003310 PC-12細(xì)胞株/細(xì)胞,大鼠腎上腺嗜鉻細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞(高分化) ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003311 MLTC-1細(xì)胞株/細(xì)胞,小鼠間質(zhì)細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003312 RM-1細(xì)胞株/細(xì)胞,小鼠前列腺癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003313 S-180細(xì)胞株/細(xì)胞,小鼠肉瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003314 HSC-T6細(xì)胞株/細(xì)胞,鼠肝星形細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003315 BRL-3A細(xì)胞株/細(xì)胞,大鼠正常肝細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003316 BRL大鼠Buffalo細(xì)胞株/細(xì)胞,大鼠肝細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003317 3T3細(xì)胞株/細(xì)胞,小鼠成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003318 Mo-MuLV/3T3細(xì)胞株/細(xì)胞,小鼠Mo-MuLv感染的3T3細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003319 3T3NIH細(xì)胞株/細(xì)胞,小鼠成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003320 PA317細(xì)胞株/細(xì)胞,逆轉(zhuǎn)錄病毒包裝的NIH3T3細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003321 L929細(xì)胞株/細(xì)胞,小鼠成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003322 L13T3細(xì)胞株/細(xì)胞,小鼠脂肪細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003323 Ana-1細(xì)胞株/細(xì)胞,小鼠巨噬細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003324 CHL細(xì)胞株/細(xì)胞,中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003325 CHO-K1細(xì)胞株/細(xì)胞,中國(guó)倉(cāng)鼠倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞亞株 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003326 CHO-pt細(xì)胞株/細(xì)胞,轉(zhuǎn)血小板基因倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003327 CHO-AA8細(xì)胞株/細(xì)胞,轉(zhuǎn)入Tet-off調(diào)控含EGFP基因的CHO細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003328 Vero細(xì)胞株/細(xì)胞,綠猴腎細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003329 VERO C1008 (E6)細(xì)胞株/細(xì)胞,非洲綠猴腎細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003330 VERO E6細(xì)胞株/細(xì)胞,猴腎細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003331 COS-7細(xì)胞株/細(xì)胞,SV40轉(zhuǎn)化的非洲綠猴腎細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003332 MDCK細(xì)胞株/細(xì)胞,狗腎細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003333 CFSC-8B細(xì)胞株/細(xì)胞,大鼠肝星形細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003334 CFSC-2G細(xì)胞株/細(xì)胞,大鼠肝星形細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003335 CFSC-3H & 5H細(xì)胞株/細(xì)胞,大鼠肝星形細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003336 WB-F344細(xì)胞株/細(xì)胞,大鼠肝上皮樣干細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003337 C2C12細(xì)胞株/細(xì)胞,鼠肌原細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003338 RLE-6TN細(xì)胞株/細(xì)胞,大鼠肺上皮Ⅱ型細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003339 L929細(xì)胞株/細(xì)胞,小鼠肺成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003340 VSMC細(xì)胞株/細(xì)胞,大鼠胸主動(dòng)脈平滑肌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003341 PA317細(xì)胞株/細(xì)胞,小鼠胚成纖維包裝細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003342 ψ2細(xì)胞株/細(xì)胞,小鼠胚成纖維包裝細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003343 BHK-21細(xì)胞株/細(xì)胞,敘利亞倉(cāng)鼠腎細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003344 ZA1細(xì)胞株/細(xì)胞,轉(zhuǎn)S9基因倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003345 ZA6細(xì)胞株/細(xì)胞,轉(zhuǎn)S9基因倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003346 MC細(xì)胞株/細(xì)胞,大鼠巨噬細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003347 COS-1細(xì)胞株/細(xì)胞,非洲綠猴腎細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003348 COS-6細(xì)胞株/細(xì)胞,非洲綠猴腎細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003349 BSC-1細(xì)胞株/細(xì)胞,猴腎細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003350 MES-13細(xì)胞株/細(xì)胞,小鼠腎小球系膜細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003351 MC53細(xì)胞株/細(xì)胞,小鼠腎系膜細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003352 NRK-49F細(xì)胞株/細(xì)胞,大鼠腎成纖微細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003353 JB6-C30細(xì)胞株/細(xì)胞,小鼠皮膚細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003354 JB6-C41細(xì)胞株/細(xì)胞,小鼠皮膚細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003355 MC-3T3細(xì)胞株/細(xì)胞,小鼠前成骨細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003356 MC3T3-E1細(xì)胞株/細(xì)胞,小鼠成骨細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
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